Hello,
We are trying to detect accurately the beta amyloid concentration of our solutions with ELISA kits, but we almost never get the concentration that we know that is been analyzed. We have tried different kits as we have read that some kits have BSA in its sample diluyent, but we still don't get it so we don't know what is the key. We prepare the beta amyloid solutions with HFIP and HEPES now, but we have already tried DMSO instead of HEPES. Anyone has any idea?
Thank you so much!
Hi María Almudena Coto Vilcapoma
There should not be any problems with using DMSO! If your problem is with the right concentrations for your solutions, I would recommend reading the following for ELISA:
https://pubmed.ncbi.nlm.nih.gov/15980612/
Article A?? Measurement by Enzyme-Linked Immunosorbent Assay
Hi,
Just to begin with, make sure that your BSA is IgG free (i got one from jackkson immuno research). Else you will get erroneous results. Also the Elisa wash buffer should be filter sterilized. These are minor things but might help getting the accurate results.
Thanks,
Alpana
- Badges
- Science topic
- Similar topics
- Engineering
- Materials Engineering
- Coating