Is there a calculator to determine the size and composition for the generated amplicons given the reverse primer, forward primer, and the DNA they were used on?
Hello Christopher Gonzales, it is so easy :
First: go to https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch
Second: enter your primer and forward sequences primer in the top box with the following format: "forwardprimernnnnreverseprimer" example: "ACATGCCGTCACCnnnnCACTTGAATTGCAC"
please bear in mind that the reverse primer must be entered as the reverse complement. You can use http://reverse-complement.com/
Third: click the blast button at the bottom and wait until the result appear.
fourth: click the top one in the result as it has the most similarity. Then just subtracting the range1-range2.
It is very helpful: https://www.youtube.com/watch?v=04t6PbopFas
If my answer was helpful please recommend it.
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