Program to find gene copy number in genome?

I am trying to find out how many copies of a gene that I have primers for are in a training set of genomes. I would like program where I enter the genome and the primer (that has degenerate...

15 November 2020 4,523 3 View

Do you have an endnote style for Marine Ornithology?

Any seabird folks out there have an endnote style file for Marine Ornithology? Nothing available online, trying to avoid creating one from scratch - thanks! Perhaps we can make the file publicly...

01 November 2020 9,642 7 View

Storing environmental data with PostgreSQL?

I am wondering if anyone uses postgresql to store data collected by continuous sensors and if there is a pipeline that makes this easier? Also, any other general tips with postgresql would be very...

18 August 2020 939 2 View

Is it necessary to do elution of proteins from Polyacrylamide gel?

Dear researchers, I would like to ask is it necessary to do Elution of proteins from Polyacrylamide gel before sending the protein sample to Nano-LC-TOF-MS Analysis? This is what I plan, I would...

01 August 2020 3,680 3 View

Aside from Mendeley, what other tools/apps can help researchers organize their citations and references?

My question is on looking for suggestions on how citations and references can be easier to generate. Thanks for the response. Hop your sharings could help me.

31 May 2020 5,026 14 View

Hi, i am conducting a legal research on; why people in Ghana are reluctant to commence tort actions like battery in court?

would be very grateful if some recommendations are made on the relevant cases or articles to read to help. thank you.

20 April 2020 4,801 3 View

Regarding on metabolomic?

Dear researchers, May I know how to know what metabolites will be produced by the cells from experiment (Wetlab): 1) Cell (cell culture) 2) Cell infected with virus 3) Cell treated with plant...

31 March 2020 5,865 3 View

How to make elution buffer for SDS-PAGE?

Dear researchers, How to make elution buffer for SDS-PAGE? There is a lot of protocol to make elution buffer, but they didn't show the amount of each ingredient needed. thank you.

25 March 2020 8,333 4 View

Elution of protein from SDS PAGE ?

Dear researchers, May I know do anyone can suggest the protocol of protein elution from SDS PAGE ? Any simple and inexpensive methods? Thank you.

24 March 2020 2,073 13 View

Regarding on Proteomics ?

Dear researchers, I'm currently doing a study regarding on antiviral mechanism of selected plant using metabolomic approach. Plant extract (fruit) will be used and the virus model will be used is...

21 March 2020 5,885 2 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Reinforcement Learning solve problems where next state is not predictable?

23 February 2021 6,870 3 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Is this 6GHz wifi antenna create complexity at the time of febrication?

I have design a 6GHz antenna for wifi.The feed line width is 2mm and there are 2 parasitic elements beside this feed line with 1 mm gap.Is there any problem at the time of febrication?

04 February 2021 9,241 3 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View