Hello to everyone. i have one question.? Can anyone suggest me suitable method except Liberman Burchard method, for Phytosterol Screening?

03 May 2016 1 533 Report

I am working on Phytochemicals.Can anyone suggest me suitable technique for screening of Phytosterols (Triterpenoids, Triterpenes, Sterols)? I have method (LB method) in which acetic anhydride is used, but due to some reasons acetic anhydride is not purchaseable. so kindly if there is any other method for Phytsterol screening, kindly share it with me or if you send article, then it would be highly appreciated.

Thanks in advance

Yogesh Chandra Tripathi

Dear Bilal,

Salkowski Test is another method for detection of phytosterols in plant extract. To 1 ml of extract solution prepared in chloroform, few drops (3-4) of conc. sulphuric acid is added. from the sides of the test tubes. Appearance of reddish brown colour in chloroform layer indicates presence of phytosterols

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