10 Questions 8 Answers 0 Followers
Questions related from Bilal Ahmad
Dear all, I am a bit confused about the RNA concentration to be used for cDNA Synthesis. I extracted RNA from 3T3-L1 cells. The 260/280 value is 2.21 and 260/230 value is 2.2 which I think is...
03 February 2022 222 6 View
Dear Scientist, I will be doing Western Blotting in 3T3-L1 cells after differentiation them to mature adipocytes. The purpose is to check the expression of some specific marker proteins...
13 September 2021 2,979 3 View
Dear scientists, I am planning to do an RT-qPCR analysis to check the expression of a few genes after treatment with my compounds. I am confused that while designing the primers, is there any...
10 March 2021 5,754 3 View
Dear scientist, what final concentration of DMSO should be used for 3T3-L1 cells? Some people say 0.1% while some reported 0.5 %? Will it be much more toxic if I use 0.5 % DMSO as a vehicle for my...
16 July 2020 8,728 2 View
Dear Scientists, I have cultured 3T3-L1 cells and checked after 24 hours. They are round in shape and attached to the flask. Below I have attached 3 pics and 2 short mobile videos of them under...
05 February 2020 3,407 2 View
Dear scientists, I have few queries. I analyzed total phenolics and total triterpenoids in both aerial and root parts of Scolymus hispanicus from asteraceae family. What I found is that phenolics...
22 October 2017 4,728 4 View
Hello dear scientists, I am following the method of L Wei, et al 2015 for the determination of total Triterpenoids contents but there is no information about the mechanism of reactions when we...
04 May 2017 6,726 3 View
I am working on Phytochemicals.Can anyone suggest me suitable technique for screening of Phytosterols (Triterpenoids, Triterpenes, Sterols)? I have method (LB method) in which acetic anhydride is...
03 May 2016 533 1 View
I am working on phytochemicals extractions. i am a little confused as to how much solvent we should use for the extraction? for example if i have 5 grams of powdered form of a sample then how much...
20 April 2016 5,513 20 View
Can any send the articles regarding ethical issues abou model organisms and experimental organisms? It would be hight appreciated. Please?
31 March 2016 8,752 2 View
