Sugars do not absorb ultraviolet light. Perform the Molisch test for identification purposes.

Method for Dextran Separation via TLC:

revert me if its works

I need it for dextran and galactose, fructose, and glucose is this a specific type of plate? Name product and help with all these saccharides

For Thin Layer Chromatography (TLC) of carbohydrates, you need to use a method suitable for polar compounds. Here's a reliable method:

Materials and Reagents

1. TLC Plate: Silica gel 60 F254 (preferred for carbohydrates)

2. Mobile Phase:

n-Butanol : Acetic acid : Water (4:1:1, v/v/v) – commonly used for carbohydrates

3. Developing Chamber: Saturate for 30 minutes before use

4. Derivatization Reagent:

Aniline-diphenylamine-phosphoric acid reagent (for color development) or

Naphthol-sulfuric acid (for general carbohydrate detection)

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Procedure

1. Sample Preparation:

Dissolve the carbohydrate sample in water or methanol (5 mg/mL).

2. Spotting:

Spot 2–5 μL of the sample on the TLC plate ~1.5 cm from the bottom edge.

Keep the spots small and allow them to dry before running.

3. Development:

Place the plate in the developing chamber containing the mobile phase.

Allow the solvent front to rise ~8 cm from the origin (this takes ~30–45 minutes).

4. Drying:

Air-dry the plate.

5. Derivatization:

Spray the plate with the aniline-diphenylamine-phosphoric acid reagent.

Heat at 110–120°C for 5–10 minutes until colored spots appear.

Alternatively, use naphthol-sulfuric acid and heat until brown/violet spots appear.

6. Visualization:

UV light (if the carbohydrate is conjugated or fluorescent)

Visible light after derivatization

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Expected Results

Different carbohydrates should separate based on their polarity and retention factor (Rf).

Monosaccharides like glucose and fructose tend to have higher Rf values than disaccharides like sucrose.

For me, ceric ammonium molybdate works best for carbohydrates (and other oxidizable compounds). You need to prepare a staining solution: (https://sarponggroup.com/wp-content/uploads/2020/01/TLCStainRecipes.pdf). Be careful when adding sulfuric acid because the solution might get quite hot. Leave the solution overnight to let everything dissolve. Once ready, dip the developed TLC aluminum plate in it for a moment (hold the TLC plate in pincers), and remove excess of the staining solution by touching the TLC plate to the paper towel (avoid touching silica) and then heat it with a heat-gun (use the fume hood). Carbohydrates decompose, leaving blue-to-dark spots (depending on the temperature applied). Make photos of the plate, as the stained and heated TLC plates do not last long.

@Ganesh yeole

The naphthol-sulfuric acis spay what kind of ethano you used 99% or 80% or what?

The dextran separation method generally uses gel filtration chromatography, especially using different types of gels such as Sephadex, Agarose and Bio-Gel. Ultrafiltration, supercritical fluid extraction, and microfluidic extraction can also be used for separation.