I am trying to study a protein and we cannot detect it until now. One hypothesis is that it is strongly regulated by miRNA, so is it possible to treat cells so that all miRNA are inhibited ?
As Zhongliang said, maybe you can use an inhibitor (Anti-miRNA) and testing in yours cells by transient transfection. You can buy miRNA inhibitor in qiagen, dharmacon, ambion, etc. All you need to know is that miRNA might be inhibiting your protein.
If you don't know which microRNA is regulating your protein, you can knockdown Drosha, DGCR8 or Dicer with siRNA or knock them out using CRISPR/Cas9 , and therefore nearly all the microRNAs should be down-regulated.