Hello; I would like to quantify the Vitamin B9 in liver of farmed fishes. Is there anyone who can give me a protocol?

More Jamel Jebali's questions See All

Did anyone have the Official Methods of Analysis of AOAC INTERNATIONAL, for fish muscle protein, fat ash, Vitamin, mineral determinations?

Researchers working on the aquaculture or in Food technology

05 June 2017 3,341 6 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Markus Christ

Article Determination of folic acid content in foods of animal origi...

Kai Bester

HPLC _MS/MS should do the trick. However it will need extraction and a bit of clean up.

Narong Chamkasem

You can google the internet to get LCMS condition from Sciex app note then combine the sample extraction of drugs or antibiotics in liver as a guide for sample preparation.

https://sciex.com/Documents/brochures/vitB_infant_formula_QTRAP6500_09340214.pdf

https://theanalyticalscientist.com/fileadmin/tas/issues/App%20Notes/0017-033-app-note-uct.pdf

you can use QuChERS extraction

http://cdn.intechopen.com/pdfs/40383/InTech-Analytical_methods_for_quantification_of_drug_metabolites_in_biological_samples.pdf