Hello;
I would like to quantify the Vitamin B9 in liver of farmed fishes by HPLC. Is there anyone who can give me a protocol?
Thanks a lot for all
Jamerl
Article Determination of folic acid content in foods of animal origi...
HPLC _MS/MS should do the trick. However it will need extraction and a bit of clean up.
You can google the internet to get LCMS condition from Sciex app note then combine the sample extraction of drugs or antibiotics in liver as a guide for sample preparation.
https://sciex.com/Documents/brochures/vitB_infant_formula_QTRAP6500_09340214.pdf
https://theanalyticalscientist.com/fileadmin/tas/issues/App%20Notes/0017-033-app-note-uct.pdf
you can use QuChERS extraction
http://cdn.intechopen.com/pdfs/40383/InTech-Analytical_methods_for_quantification_of_drug_metabolites_in_biological_samples.pdf
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