The assay of SOD employs xanthine and xanthine oxidase to generate superoxide radicals which on reaction with 2-(4-iodophenyl) -5-phenyltetrazolium chloride (INT) forms a red formazan dye. The absorbance is measured at 505nm by a spectrophotometer. SOD is measured from the degree of inhibition of this reaction and expressed as units/ml. One unit is equal to the amount of sod to cause 50 percent inhibition of reduction rate. For details, my paper on serum SOD as tuberculosis diagnostics may be gone through.

Please reed the attached paper and dont hesitate to contact me for further informations.

I.N. Popov

Please reed the attached paper and dont hesitate to contact me for further informations.

I.N. Popov