1- After 9 days of siRNA transfection, I'm required to gather samples, however I'm not sure if this is okay or if the effect of the siRNA would be weakened.
2- Which is it better for CMs, reverse or forward transfection?
I am looking for professional software to draw the physical structure of nanoparticles or vesicles, not the chemical structures for which we use ChemDraw, but the actual physical structures,...
23 June 2024 1,292 3 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
Hi, I've observed a new biological activity in certain cells, likely induced by an enzyme. How can I determine the gene or genes responsible for expressing this unknown enzyme? Thanks in advance
31 May 2024 9,111 3 View
I have many skills like data analysis with R programming or Spss , scientific writing and paper publishing
23 May 2024 1,683 3 View
I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...
16 April 2024 3,537 2 View
The positive curves for E coli different strains from mucual stool sample.
29 March 2024 5,907 0 View
methylation of spata 6 gene and its role on male infertility of rat
08 March 2024 9,667 0 View
how to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?
04 March 2024 1,822 0 View
I am a researcher who decided to buy labtop suitable for my work as a simulation and data analysis work Please suggest one
23 February 2024 3,635 2 View
Note: Serum isolated extracellular vesicles using immunoprecipitation (magnetic beads)
20 February 2024 4,894 1 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View