Alaa Kaoud

12 Questions 9 Answers 0 Followers

Questions related from Alaa Kaoud

What is the most accurate method to measure intracellular NAD level?

I am trying to measure intracellular (in vitro) NAD using EnzyChrom NAD assay kit, however the results are variable. I was wondering if cell dissociation could affect its level. Are there tips and...

21 November 2024 8,339 2 View

How could I confirm good quality of samples for RNA sequencing ?

I collected samples for gene over-expression in iPSC-CM. I confirmed significant over-expression by pcr and confirmed good induction efficiency before conducting the analysis However, in PCA the...

07 August 2024 6,833 2 View

How to interpret the results of seahorse?

What does it mean when I have coupling efficiency higher then 100% and proton leak zero? I did seahorse assay for the first time, and I'm not an expert in that field. How can I know that the...

18 July 2024 517 0 View

Does gene expression stability could have an effect on the results of viability assay?

I am doing viability assay after gene overexpression. For overexpression, I used AAV vector and checked the gene expression using RTqPCR. Although, it was statistically significant compared to the...

05 July 2024 8,748 3 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

03 June 2024 7,887 2 View

Does GFP interferes with absorbance in MTT assay or luminescence in Caspase 3/7 Glo assay?

I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...

17 April 2024 3,579 2 View

Hello, how could I transfect cardiomyocytes with siRNA and get the target efficiently?

1- After 9 days of siRNA transfection, I'm required to gather samples, however I'm not sure if this is okay or if the effect of the siRNA would be weakened. 2- Which is it better for CMs, reverse...

16 February 2024 2,114 0 View

What causes cell detachment after replating into a monolayer?

I dissociated EBs and replated hiPS-CM into a monolayer in a fibronectin coated plates. I had some cells in 96-well plate and others in a 12-well plate. After 24 hrs., cells became adherent and...

08 February 2024 8,978 1 View

When iPS cells should be discarded?

I want to know, when I need to discard my iPS cells, despite they show good differentiation efficiency? What could I see under microscope?

27 November 2023 2,281 2 View

CM induction and detection of TNNT2 level?

I did induction of iPScells to CM. Then I did sort these CM and divided into two groups for a target experiment. I checked the TNNT2 level by qPCR, however, it varied between both groups. I am not...

26 October 2023 3,714 0 View

How to improve induction skills?

I am new in the field of iPS cells. I am practicing induction of iPS cells into cardiomyocytes via embryoid bodies. I have difficulties to improve my induction skills. Could you tell me tips and...

02 October 2023 9,548 2 View

Could you please provide me with Molecular Biology of the Cell" by Bruce Alberts manual?

I want the manual of molecular cell biology by Bruse Albert

27 April 2023 3,405 3 View