8 Questions 5 Answers 0 Followers
Questions related from Alaa Kaoud
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
03 June 2024 7,780 2 View
I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...
17 April 2024 3,511 2 View
1- After 9 days of siRNA transfection, I'm required to gather samples, however I'm not sure if this is okay or if the effect of the siRNA would be weakened. 2- Which is it better for CMs, reverse...
16 February 2024 2,060 0 View
I dissociated EBs and replated hiPS-CM into a monolayer in a fibronectin coated plates. I had some cells in 96-well plate and others in a 12-well plate. After 24 hrs., cells became adherent and...
08 February 2024 8,930 1 View
I want to know, when I need to discard my iPS cells, despite they show good differentiation efficiency? What could I see under microscope?
27 November 2023 2,229 2 View
I did induction of iPScells to CM. Then I did sort these CM and divided into two groups for a target experiment. I checked the TNNT2 level by qPCR, however, it varied between both groups. I am not...
26 October 2023 3,649 0 View
I am new in the field of iPS cells. I am practicing induction of iPS cells into cardiomyocytes via embryoid bodies. I have difficulties to improve my induction skills. Could you tell me tips and...
02 October 2023 9,507 2 View
I want the manual of molecular cell biology by Bruse Albert
27 April 2023 3,365 3 View
