Ambrose Teru Patrick

4 Questions 2 Answers 0 Followers

Questions related from Ambrose Teru Patrick

Hello everyone, please can somebody assist me here.?

I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you I want to crystallize my protein with this buffer? i need 0.1M working...

04 April 2024 9,994 0 View

Cloning with pAcGP67A vector with GP64A signal peptide?

Hello everyone, Is there anyone who has cloned with pAcGP67A secretion vector with GP67A signal peptide. I tried to clone with insert 1.2kb in the vector, MCS: BamHI and NotI. C-term Thrombin and...

13 December 2023 5,077 1 View

Hi, please i need a help. Attached is my Agarose gel containing ligation reaction (pET28a) and VEGFA (insert).?

Hi, please i need a help. Attached is my Agarose gel containing ligation reaction (pET28a) and VEGFA (insert). After ligating for overnight at 16 degree and run gel with 10uL from the ligation...

29 November 2023 3,505 3 View

Hello everyone, please can somebody assist me here.?

I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you I want to crystallize my protein with this buffer? i need 0.1M working...

01 January 1970 9,821 0 View