Hello everyone, may I get the information about the optimal molecular weight of dextran used to get the pure isolation of neutrophil?

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La-Ongthip Rak

I used 3% dextran in NSS for PMN isolation (there is dextran powder in the research 3). You can ask Meej about it.

hope this help

Rotme

Pyae Mon Kyaw

Thank you so much P’.

Caroline A Owen

We use a 6% solution of dextran in normal saline in order to sediments the red cells. The molecular weight of the dextran doesn't matter.

Guillermina Calo

Caroline A Owen what do you mean molecular weight doesn't matter? is it the same to use a 300,000 Mw or a 500,000 ?