I have done DGGE gel analysis for three different genes from ten rhizosphere plants. I have got the data on band intensity and SW index and I would like to do Principal Component analysis (PCA) with SW index alone for ten samples along with all three genes in a single PCA analysis. Is it possible to do PCA with the available data and also any software that can be easily used for the same. Need help in this.
Hi Baskaran,
If you're trying to do PCA on a series of images, check out: http://biochem.missouri.edu/trend/
If your data is a numeric matrix you can use my webserver: https://beamerlab.shinyapps.io/software/
The webserver expects data in the format 'ID | Variable1 | Variable 2| etc' with the first row of the CSV being labels, first column being identifiers, optional 2nd column of groupings, and the following columns all being numeric data. If you don't have groupings in your 2nd column, make sure to change the slider to "2".
Good luck!
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Statistics
- Statistical Software
- SPSS