Ambra Annibali

2 Questions 2 Answers 0 Followers

Questions related from Ambra Annibali

GSHt Assay with Ellman' reagent. Can I add the GR+NADPH later and measure the GSSG too?

Hello. I am new to the GSH assay and I am learning about it online. I performed it this week for the first time. My protocol said to add DTNB and GR to my GSH standards + samples - allow 30 sec...

25 January 2019 8,330 1 View

Hello, does someone has a protocol for GSSG measure in ES cells (or cell lysate anyway)?

Trying to measure the GSH:GSSG ratio in my ES cells. I found several protocols for both GSH and GSSG measure, but mostly from blood or tissue. Not sure what to choose for my purpose. Does someone...

26 November 2018 8,544 3 View