Are there other relevant techniques other than swabbing method? (maybe dilute with some solutions). Swabbing may require hair shaving which might alter microbiota composition of the skin.
What is the highest EQE ever achieved by a light-emitting diode containing a multiple quantum well structure? I'm looking for record values achieved for different colours / wavelength regions...
10 August 2023 5,008 2 View
Good afternoon. I want to introduce my self, I am Dede Kosasih from Sundanese Language Education Study Program, FPBS UPI Bandung. I apologize for permission to submit questions and suggestions,...
02 April 2023 1,416 3 View
I'm currently writing my dissertation (3rd year adult nursing student) on the impact of patient nurse ratio in the UK, and have been researching around Australia and California where there are...
17 December 2022 8,316 6 View
Lineage tracing can be done by activating reporter gene which is positioned next to stop codon. Some people use cre-lox system as the reporter transcription controller, the others favor...
20 June 2022 8,474 0 View
1. In case of Cre-ER-flox using flox cutting system, will the daughter cells will always carry the transgene no matter how much they divides? (the parent cell has been flox-activated by...
14 June 2022 351 0 View
Mice pups are very small, could it be given through oral gavage? or are there other methods which are more suitable? Thanks
15 January 2022 3,080 3 View
i have extracted bacterial genomic DNA, and after running multiplex PCR on it, i got blurry thick bands below marker. the marker used is 100 bp. i checked using nanodrop and diluted the DNA conc...
26 April 2018 8,957 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View