Hello everyone,

I am running a western blot for pSTAT3 (Santa Cruz antibody, host: mouse). recently (three days ago) I started to see a strange, aspecific band, which is equal in all lanes and size corresponds to heavy chain. My western is on total extracts from cells, not on IP. I did not change any conditions. Secondary antibody is of course anti-mouse. I thought it might be something in my samples (they were all from one experiment) but the second time I included as a control a sample from a previous experiment which gave only the correct band before, but now also shows the aspecific one. Has anyone ever seen anything like this or maybe has any ideas how to explain this?

Kris

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