I am profiling miRNAs using SYBR Green real-time PCR. Some of the miRNAs show only a linear amplification curve without any exponential part. In these cases, the amplification starts at very early cycles. Does anybody had a similar experience? I tried different RNA/cDNA concentrations with the same result. In the array system which I use, the primer and cycle conditions are optimized by a company.
better to provide a "print screen", otherwise "linear amplification" seems like you have no amplification at all.
Hi Andrey,
Thank you for your notification, I added two screenshots. I hope they will explain my concern.
Hi Földes Katalin ,
Melt curve is OK or no amplification at all? Usually, when i got some curves like that, i had no amplification. If some samples did not amplify, probably you have low miRNA expression in these samples.
Best,
Jacó
Hi Jacó,
Thank you for your answer. Usually in those samples, the melting curve is problematic or negative. Did it happaned to you this in internal control samples?
- Badges
- Science topic