What is the experimental water-octanol partition coefficient of cholesterol?

Please, send a paper on this subject If you have or know about It.

08 September 2018 8,208 0 View

Can anyone suggest a literature about small interference RNA (siRNA)?

I am interested in leaning about the structure prediction and design, molecular mechanisms and biological functions of siRNA, gene silencing (in special the PTK7 gene), and transfection using...

07 August 2018 3,214 6 View

What is the most suitable PEG for encapsulating peptides in order to reach the alveoli regions by inhalation?

I would like to make PEG-peptides nanoparticules to reach the alveoli regions by inhalation.

06 July 2018 2,594 1 View

What is the reproducibility of the umbrella sampling for calculating free energies in order to estimate the oil-water partition coefficient?

The calculation of the free Gibbs energy of transferring a molecule from the aqueous phase to octanol phase (or vice versa) by using umbrella sampling in order to estimate the oil-water partition...

05 June 2018 2,023 0 View

How to build reduced coarse grained models for phospholipids with two ir three beads?

Please, tell me any good paper or web site to download models.

04 May 2018 6,176 1 View

What is the best suitable method for measuring the partition coefficient logP (or distribution coefficient, logD) for ionizable compounds (drugs)?

Potentiometric or HPLC?

04 May 2018 8,296 0 View

Have you worked with Naringenin and Naringin as antimicrobial agents? What is the effect of their interaction with lung surfactant and bacteria?

I am interested in getting more information about this subject.

03 April 2018 4,114 0 View

How can I perform error analysis in umbrella sampling simulations?

Do you have any program to evaluate the statistical error in umbrella sampling?

01 February 2018 3,488 0 View

Has anyone encapsulated antimicrobial peptides for drug deliever to lung?

Please, let me know any procedure or paper about this subject.

07 August 2017 1,371 3 View

What are the best materials for drug encapsulation to deliever into Lung?

Please, send me literature about It.

07 August 2017 9,939 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View