Have you work experience from Cytation 5 system to luminescence measurement?

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What is the good dilution factor for mouse serum in ELISA?

I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.

28 April 2024 8,922 2 View

Which type of glue can be used for peeling test?

I want to do peeling test on commercial cathode and anode electrode. Is there special glue tape for testing?

28 November 2023 8,548 2 View

Real-Time PCR Troubleshooting: Amplicon Identity, Curve Overlap, and Melting Temperature Discrepancies?

I am encountering several issues in my real-time PCR experiments and seeking guidance on troubleshooting: 1. Inconsistent Amplification Curve Overlap: The amplification curves in my real-time...

08 October 2023 4,939 0 View

Does anyone have experience using Forced Intercalation-Peptide Nucleic Acid Probes (PNA-FIT) labeled with thiazole orange dye for qPCR?

The first question is, should they take out of the lyophilized state with a special solution such as formamide? Based on the experience that we used to remove the TaqMan probes from the...

05 October 2023 6,532 0 View

Variability in cDNA Synthesis and PCR Results: Impact on Real-Time PCR?

I recently conducted cDNA synthesis followed by conventional PCR for quality assessment. In the course of this experiment, I observed some unexpected results that have raised questions about the...

09 September 2023 1,020 5 View

How is mRNA extracted from hydrogel film-treated L929 cells in a purified manner?

How is mRNA extracted from hydrogel film-treated L929 cells in a purified manner? And at what stage is it preferable to extract it after exposing the cells to treatment for a period of 24 AH, 48...

18 August 2023 9,992 0 View

Could anyone provide me a complete protocol of IL6 mice plasma ELISA?

I've been measuring IL6 of mice plasma through ELISA but facing poor ODs for my samples, however standard curve and company controls are pretty fine.

13 July 2023 7,068 4 View

Smear and Absence of 28s and 18s Bands in Total RNA Electrophoresis, What Can I do?

During the electrophoresis run on a 1% agarose gel at 100 volts for 25 minutes, I was unable to clearly visualize the expected 28S and 18S bands representing ribosomal RNA. Instead, a smear-like...

27 June 2023 532 5 View

What could be the cause of the high A260/A280 ratio (2.48) and the very low-strength 28s and 18s bands observed in the agarose 1% gel electrophoresis?

I am currently facing a challenge related to the extraction of total RNA from 40 mg colon tissue, and I would greatly appreciate your expertise and insights in troubleshooting the issue. During...

19 June 2023 9,532 3 View

Residual standard deviation for XPS spectra?

How to calculate residual standard deviation (RSD) between between the actual XPS spectra and the total fitted data? please guide me Thank you

08 June 2023 2,613 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View