1) Do you count discrete lesions (low-grade, high-grade, CIS, SCC) per section? Per tongue? Anterior, middle third? Or do you simply assign the highest grade to the tongue as a whole? I have done the former.
2) Where have the majority of your lesions developed? The literature is not consistent (tip-of-tongue versus posterior tongue)
So, I did not get any hits on this but here are some general findings:
1) posterior tongue is much more susceptible to SCC than other areas when rats are admin 4NQO1 in the water. These lesions tended to be large and quite invasive. Because they undermined the epithelium, there were what appeared to be "islands" of SCC away from the surface dysplasia. I did see frank perivascular invasion, which I have not seen in the hamster model of SCC (a VERY different model).
2) mild epithelial dysplasia is difficult to discern between normal tongue mucosa due to the architecture of the anterior two thirds of the oral tongue in the rat.
3) Two pathologists (one resident) had good agreement in distinguishing low-grade (mild & moderate) v high-grade (severe and CIS) vs SCC.
4) Because of the size of the tongues, reading the slides took a considerable amount of time. Approximately 10 minutes per slide x 200 slides. Plan accordingly.
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