Have anyone done skim milk preservation for fungus?

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Alaa Kareem Niamah

Hi,

Look in attach file

Gamal Enan

Sterile milk is lyophilized and this powder must be stabbed with fungal spores.To collect spores, fungi are grown in suitable medium for 5-7 days and then centrifuge for 15 min. at 5000 rpm.The supernatant contain the spores and from this add to the powdered skim milk and store in deep freez at -20.

Chibundu N Ezekiel

Hi Haslinda,

Normally, the spores of mould are mixed in sterile skim milk and then placed on silica gel for cold storage. You can check the links:

https://www.atcc.org/~/media/PDFs/Technical%20Bulletins/tb02.ashx

http://www.microbiologyresearch.org/docserver/fulltext/micro/58/3/mic-58-3-423.pdf?expires=1544696333&id=id&accname=guest&checksum=74E127BF3889DE09EE01CFEEE325A2F5