Have anyone carry out protocols for estimate DNA methylation on PCR ?

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Mark Ziemann

There are a number of ways to determine DNA methylation of specific loci with qPCR:

Methylation specific PCR

Article Methylation-specific PCR

MBD-CAP qPCR (similar to MeDIP-qPCR) https://assets.thermofisher.com/TFS-Assets/LSG/manuals/methylminer_man.pdf

MSRE-PCR

Article MSRE-PCR for analysis of gene-specific DNA methylation