Hi,
I want to extract DNA from dried cacao leaves. In the kit DNeasy Plant Pro it is recommanded to add a buffer in the tube for the disruption with the TissueLyser. Meanwhile it is not recommended in the documentation of the TissurLyser to add a buffer for the disruption for dried leaves.
When I have tried with the buffer, no disruption has happened.
What could you advise for extraction of DNA with dried leaves ? Buffer or no buffer ? TissueLyser or FastPrep ?
Hi Anne-Sophie, I'm having this same problem - did you find the solution?
Hi Zoe,
I have stopped this quite a while ago. But, my conclusion was that when I was doing the lysis withour buffer the disruption was better (so the yield too) but there was more shearing. The best technic seems to be grounding with mortar and pestle with liquid nitrogen. And you have you done some progress?
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