Anne-Sophie Bouchon

4 Questions 6 Answers 0 Followers

Questions related from Anne-Sophie Bouchon

How to prevent degradation of –(-)Epicatechin from cacao in the lab?

Hi, I want to measure –(-)Epicatechin from cacao by fluorimetry. I have used acetone, water, acetic acid (70%, 29.5%, 0.5%) and sonication for the extraction. I have to let the sample evaporate...

08 August 2019 6,440 4 View

How to extract elemental sulfur in plants but not total sulfur?

Hi, I would like to measure only elemental sulfur in plant tissue with MP-AES. How could I do that? Protein precipitation might not be the solution because there wouldl be still amino acid in the...

07 July 2019 5,421 1 View

Has someone tried DNeasy Plant Pro with dried leaves and TissueLyserII?

Hi, I want to extract DNA from dried cacao leaves. In the kit DNeasy Plant Pro it is recommanded to add a buffer in the tube for the disruption with the TissueLyser. Meanwhile it is not...

11 November 2018 963 2 View

How to remove PVP into enzymatic extracts

Hi, I have extracted enzyme (PPO) in phosphate buffer and PVP (15g/L) and then centrifuge (12,000 g, 10 min). This seems this concentration seem to inhibit the PPO. So, I want to remove it to...

01 January 1970 8,945 5 View