How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Software for Network Analysis?

Hi everyone, I am looking for some advice on what to use to perform a network analysis. Specifically, I want to evaluate the frequency/strength of connections between country collaboration on...

15 November 2020 401 3 View

What is the problem with my 10um Nissl stained cell?

Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...

07 November 2020 9,568 3 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

Not sure what does 3mg/kg mean in AD STZ injection?

What I searched the paper online they mentioned do 3mg/kg ICV on mice On Day 1 and Day3. Doese it mean total 3mg/kg for 2 days, or 1.5mg/kg for day1 and another 1.5mg/kg for day3? Help help to...

19 October 2020 8,687 3 View

How to analyze porous granules with SEM ?

I need to analyze the cross-section surface of highly porous granules with the SEM but I don't know how to prepare the samples. Is there anybody who have an idea of how to flatten porous and...

07 October 2020 4,861 9 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Do anyone know a good software allowing interactive 3D visualization of density plot?

Hello, I would like to make a interactive 3D representation of some data point. We are studying position of some cell of interest and we extract from serial coronal section XY position of the...

15 June 2020 8,117 2 View

No title

14 June 2020 2,475 7 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View