Louis Szeponik

3 Questions 7 Answers 0 Followers

Questions related from Louis Szeponik

Has somebody successfully used Tyramide in frozen human tumor tissue?

Hej, I have been using Tyramide a lot for both human FFPE and frozen mouse tissues. I have had always good results with these tissues. I am struggling to use the Tyramide systems in frozen human...

17 September 2019 1,230 1 View

Good protocol for flowcytometry staining of T-Bet in human colorectal cancer tissue?

Hej, we are struggling with T-Bet flow cytometry staining for lymphocytes from human colorectal cancer tissue. We are getting a good staining from PBMCs but no positive cells from the tissue. We...

18 October 2018 2,115 2 View

Anybody experience with Tyramide Signal Amplification (TSA) in frozen tissue sections?

Hej, has anybody experience with TSA in frozen sections and has also seen background/unspecific signal problems? I have used TSA from Perkin Elmer and ThermoFisher in FFPE sections and it always...

25 May 2018 2,626 2 View