Quartz crystal microbalance is the best method to detect or sense micro molecules such as DNA, RNA, protein and microorganisms like bacteria and fungi.
Yes and some of my colleagues as well. Can you be more specific in the question?
Yes, we have developed extremely sensitive gas sensors based on QCM probes. Only in the gas phase they will show their ultimate sensitivity. In liquids they are heavily damped by the viscosity of the solution and its temperature dependence. Also the specific density of the sample liquid will influence the frequency shift. It is nearly impossible to keep the viscosity and the density of every sample fluid totally identical to the 5th. position after the point.
To make ist clear: Is it rather impossible to keep the ultimate sensitivity of the QCM in liquid samples. Some orders of magnitude are lost. A leading institute in Germany tried to develop a HIV biosensor with mass sensitive transducer several years ago. Despite it was even mentioned in TV, it never entered the market due to missing reliability. A Canadian researcher even detected a mass loss after the binding of one immuno partner.
Sir I want to immobilize protein to qcm probe then chek sensitivity. Will it Work?
The Attana qcm system is developed to characterize molecular interactions, providing information about kinitects, affinity,, concentration etc. You can work with both biochemical assays and cell based assays. See more on www.attana.com
Dear Parul,
The Quartz crystal cleaninig will be done using boiling mixture of 35% of
ammonia, 33% of hydrogen peroxide and deionized (Milli-Q) water
(Millipore, 18.2Mcm) in a ratio 1:1:5 for 10 min.
The procedure will give you clean working surface.
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