After sequencing, we got really low-quality data with a Phred score of
I am new to Abaqus modelling and for studying I am modelling a damper seen in a research paper. I believe I have modelled it correctly, but when I run the analysis the model shows barely any...
11 April 2024 2,619 4 View
Salt formation of weak acid causes ionization of drug due to which solubility increase but we have studied drug absorbed in unionized form then how salt formation will improve the absorption of a...
08 February 2023 5,475 5 View
As per Modified noyes whitney equation (dc/dt = DAKo/w (Cs-Cb)/Vh), rate of dissolution is directly propertional to Partition coefficient. But we have studied partitiion coefficient means...
08 February 2023 1,722 3 View
i have initital volume of supernatant of 100ml, after pricipitation with 80% Ammonium sulfate saturation in how much buffer should be used to resuspend pellet?As the initital volume is 100ml if...
21 November 2022 4,088 2 View
Which is better in between nanoemulsion and SMEDDS / SNEDDS?
06 August 2022 2,356 1 View
Hello every one . i am developing a varnish for application in dentistry. can some one pls suggest test to evaluate ,physical and mechanical properties. these test are conducted for paints and...
23 March 2022 8,559 2 View
I hve read many research papers in which drug release through dialysis bag was less than release through rat intestine? So please suggest me what may be the reason behind it?
03 March 2022 8,344 4 View
What can be the reason for small or not sharp peak in DSC ( differential scanning colorimetry) of a pure drug
08 January 2022 1,285 3 View
Today IoT devices are required network security i want to perform some research work in this area so kindly provide Best open source tool for Artificial Intelligence IoT Network Security...
18 April 2021 9,766 7 View
Can we perform trial sequential analysis in meta-analysis by using R? If yes, what codes to be used? Thanks
02 January 2021 3,774 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
I have synthesized a gene specific primer for amplification of my target gene. However, I am getting amplicon of only 100 bp rather than 210 bp (target amplicon size). So far I have tested so many...
05 June 2024 1,587 7 View
my gene of interest size is about 4kb. I used hi fidelity taq pol as well as Q5 from NEB but still its happening. my forward primer composed of a 4 adenosine then restriction site followed by 6x...
05 May 2024 4,558 2 View
I recently designed the qrtpcr primers from the 3'utr of the plant's genomic DNA keeping in mind that the annealing temperature should be 60 degree. Once I received the primers first to optimise...
25 April 2024 5,935 3 View
Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...
23 April 2024 6,637 0 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
09 April 2024 4,487 5 View
Hello! I am trying to characterize communities of eukaryotes living in biofilms using V4 region 18s amplicon sequencing data. Working with this type of data is new to me, and very interesting!...
04 April 2024 6,603 1 View
Hello, I am currently attempting to obtain amplifications of SoxC from a species of onychophoran belonging to the Peripatidae family using primers designed from a sequence of its sister family,...
20 March 2024 4,626 4 View