Tareg Omer Mohammed

5 Questions 20 Answers 0 Followers

Questions related from Tareg Omer Mohammed

How to make tumor size measurements more objective?

Greetings, These days working on s.c. tumors implanted on mice and felt like the process of tumor size measurement has much subjective element in it. I use calipers to measure the longest side of...

05 May 2016 5,222 5 View

When doing ELISA on cell lysate or supernatant, how importnat is it to measure total protein?

I am doing an experminent on human monocyte derived macrophages to see profile of cytokines after stimulation with a MoAb by doing ELISA on both supernatant and lysate. Is it necessary to measure...

09 September 2015 2,311 7 View

Which detection antibody would be most appropriate to carry coupling efficiency test of microsphere beads coupled to Mouse Anti-human Gal-9 mAB IgG2b?

As the capture antibody is mouse antihuman, I suppose I will need to use antiseptics detection antibody such as rabbit anti mouse, goat anti mouse. I could infer this from answers I received...

10 October 2014 1,528 2 View

Does it have any unfavorable effect to use PBS, 1%BSA as a wash buffer for a luminex based immunoassay?

I was wondering if it would create any problem using a PBS, 1%BSA buffer as a wash buffer for a luminex based immunoassay. So far I have been using a wash buffer that comes as part of a Bio-Plex...

09 September 2014 8,114 6 View

Is it okay to use the same capture-detection antibody pair that is used for the assay to determine coupling efficency of luminex magnetic beads?

I am working on developing a luminex sandwich immunoassay and discovered that the literature (luminex xMAPCookBook) recommends carrying out a coupling efficiency test. I used the same antibody...

08 August 2014 2,984 3 View