Has anyone worked with AC 264613 (an agonist that activates PAR2 receptor, from R&D Systems) and knows at what concentration it activates PAR2 receptor causing intracellular levels of Calcium to increase?
Or any documentation that I could use as a reference? I will be treating tracheal primary cells with this agonist, in order to measure intracellular levels of Calcium, and I would like to know at which concentration there could be a response.
*AC 264613 is analog to tryptase; stimulates PI hydrolysis, Ca2+ mobilization and cellular proliferation in vitro.
Hi Frances, we have included references for the use of this compound on the product page on the R&D and Tocris websites. According to Gardell et al., JPET 2008, the EC50 for calcium mobilization is 100nM (pEC50=7) (see Table 1 and Figure 4 in the reference). Hopefully this helps you to decide on the appropriate concentration range to use. If you have further questions our technical support team would be happy to help, you can drop them an email using the address listed on our website. Best wishes,
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