Has anyone used Triple-SVM classifier for real and pseudo-miRNA classification?

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Has anyone used Couplet-syntax for pre-miRNA prediction??

I am working on microRNA informatics..!! I have downloaded couplet-syntax; but there is no help manual available with it.!! Anyone knows whether this program takes sequence-secondary structure...

03 April 2016 5,931 0 View

How can I assign seed type to novel predicted miRNA (eg:7mer1A or 8A) ???

Is there any way to find seed sequence of predicted miRNA which is novel (not reported in literature).

02 March 2016 8,203 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

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E.coli contamination in human RNA seq data ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

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Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

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How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Isha Monga

@BK Bhowmick its a feature for real and pseudo-pre-miRNA discrimination.Thsi feature can be implemented in nucleotide sequences having secondary structures..

by the way; thank you for your concern. I have solved the problem..

Shuichi Shinmura

In my research, SVMs are not helpfull for feature selection of microarray. Download my 16 papers after last Oct.

Pradyumna J.

Hi, If you get the downloadable link for Triple-SVM, can you share the linkplease?

Thank you.