Has anyone used the Lonza Primary hepatocytes ( plateable) for drug studies? if so, do you strictly follow the Lonza protocols ( seeding density, culturing conditions etc). When you thaw the vial, how many cells do you actually get? And do you immediately plate it? Lonza claims these cannot be subcultured as they are non-proliferative. Is this what you found too? These cells are really costly and we would like to do two sets of experiments ( qPCR and cytotox assays) using one vial, if possible, as affording another one is really tough. Any help is greatly appreciated.