Bhaskar Chatterjee

5 Questions 8 Answers 0 Followers

Questions related from Bhaskar Chatterjee

Has anyone done transfections on HLEB3 cell line successfully?

I need to transfect some plasmids in the HLEB3 cell line but I do not find evidence that this was tried, apart from the transfection of SV40 T antigen in primary HLE cells. If anyone has done...

14 August 2021 251 3 View

Failure with Gibson assembly for one fragment assembly (5.7 Kb backbone with inserts varying between 0.9-1.5kb) ?

I am trying to perform a hifi assembly(NEB HIFI master mix) of a backbone (5.7kb) and inserts ( 0.9 - 1.5 kb) in a single fragment reaction assembly ( a different construct corresponding to each...

16 May 2019 9,881 3 View

Why mP values in a FP assay are decreasing?

I initially did an FP assay with receptor (A) and a flourescent labelled ATP analog (B). For the first three times, i tried varying B conc for a fixed A concentration. I found increase in mP...

08 April 2019 3,721 1 View

I am planning to use cycloheximide for inhibiting protein synthesis in HeLa cells. What concentration should I use?

I need to know what concentration of cycloheximide can be used in HeLa Cells without killing the cells. According to Santa Cruz Biotech that sells this compound, IC50 value of CHX is 532nM for...

24 November 2015 1,014 5 View

Does heat shock to Hela cells produce certain chemicals or changes in cell morphology that affect the MTT assay?

After a certain incubation period at high temperature, I am getting higher MTT readings as compared to smaller incubation periods at the same temperature. Cell viability should ideally decrease...

21 May 2015 149 2 View