Works well? Any recomendation ?
Hi Alexandra Targovnik
We use MEGAscript kits from Invitrogen, Works well, high yield and good quality
https://www.thermofisher.com/order/catalog/product/AM1333
I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...
25 December 2020 6,258 4 View
You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...
11 November 2020 9,715 1 View
Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...
21 October 2020 2,182 5 View
I was able to find some applications of deep watershed for image instance segmentation, but nothing for rocks. Could you please recommend any study?
07 September 2020 5,731 8 View
I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...
04 August 2020 4,446 7 View
Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon
27 July 2020 7,302 3 View
Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...
08 July 2020 5,926 5 View
At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...
22 June 2020 8,144 6 View
What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method? Can the EEG record accurately activity in deeper brain structures...
11 March 2020 1,087 6 View
Covid-19, apart from all health consequences, is also having an impact on research projects with human participants. Universities and schools are shutting down and, even when labs are still open,...
11 March 2020 2,711 16 View
I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...
08 February 2021 2,032 2 View
Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...
31 January 2021 2,516 1 View
Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...
16 December 2020 5,795 3 View
Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...
13 December 2020 942 3 View
I would like to find off target sites for my sgRNA, however the more commonly used off target finders do not include apis meliifera, is there an application or website that would allow me to do...
09 November 2020 3,463 1 View
I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...
03 August 2020 2,854 4 View
Hello - I am studying this protocol which claims to use pcr to clone in/replace a small piece of an sgrna plasmid with a new guide sequence provided by an oligo with homology to the sgrna...
02 March 2020 6,358 2 View
I am using the following protocol from a CSH lab manual edited by Doudna on CRISPR, to attempt to retarget sgrna scaffold in a plasmid. but....I get DNA stuck in the well, not migrating....
01 February 2020 6,327 3 View
I am currently trying to generate CRISPR sgRNA pairs with Sanger Institute's online genome-editing tool. I enter the sequence of interest and it generates hundreds of sgRNA pairs, which I can rank...
09 October 2019 4,289 2 View
Currently, I am using a plasmid containing two gRNA cloning site. The restriction enzymes used for cloning gRNAs into this plasmid, pX333, are BsaI and BbsI. I digested the pX333 plasmid using...
09 October 2019 2,743 6 View
