Has anyone used the EnGen sgRNA synthesis kit from NEB? What did you do with your RNA product (how did you transform it into your cells)?

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Jai Ghosh

I think CRISPER/Cas 9 works better with prokaryote rather than a eukaryote.

Mahmoud Kamal Ahmadi

You can try endogenously expressing systems. One example is pCRCT plasmid that allows you to express the sgRNA and Cas9 in one plasmid. It works fine for yeast systems but not sure for fungi. Here is the link to the paper:

https://www.ncbi.nlm.nih.gov/pubmed/25207793