Can someone send me questionnaire about parental pressure impact teenage performance and metal skill in football?

2) How does parental pressure impact teenage performance and metal skill in football.

13 December 2020 3,586 1 View

How do I make sure that all levels of a factor is resampled evenly during a train / test portion of LDA in R?

I am attempting to create train and test data for an LDA. I keep getting an error message that my test and train data have different levels of different factors for one of my factors. Here is the...

16 June 2020 8,968 3 View

Syncing data with ultrasound in Vicon system?

I am looking to sync data across an ultrasound machine (Terason USmart 3300), Vicon motion capture, force plate, and Delsys EMG data. I have gotten the Vicon, force plate, EMG to sync within a...

11 February 2020 580 3 View

How to control for effects in SEM model?

I am testing a model with 1 IV, 1 DV, and 4 mediators of these. In Mplus, how can I control for the other mediation effects while independently estimating one mediation/indirect effect?

17 September 2019 6,328 3 View

Comparing HPLC traces between different samples?

I have been assigned a task and I am a little bit out of my comfort zone. I am looking for software recommendations for HPLC statistical analysis that can incorporate several factors/indicators...

09 January 2018 3,377 3 View

What dose of pyridoxine is recommended for nausea and vomiting in pregnancy?

I have read the recent withdrawal of the recommendation to use Diclectin for morning sickness. Pyridoxine is recommended in its place. Diclectin contains 10 mg, but the smallest dose of pyridoxine...

31 January 2017 9,679 4 View

What needs to be added to culture media for neutrophil phagocytosis assays?

I am performing neutrophil phagocytosis assays (with pHrodo bioparticles), and need to incubate the neutrophils for 2 hours in culture media before the assay. I am using RPMI with L-glutamine, and...

02 November 2016 6,003 2 View

What DNA extraction kits/methods do you use that produce good enough quantity/quality DNA to run Agilent arrays?

I have tried Gentra, QIAamp DNA Mini, and iGENatal v2. Gentra and iGENatal give good quality but poor concentrations/yield. QIAamp gives decent yields but poor quality. Goal is 2 ug at 20 ng/uL...

18 November 2015 7,040 2 View

Is there a special lysate protocol for eye tissue in Western blot?

I've started a brief foray into Western blots. My PI and I have prepared our samples from eye tissue of zebrafish. When we performed the acetone precipitation steps (which we did three times), a...

16 September 2014 1,767 2 View

Could Social Media Play an Important Role in Vietnamese Health Promotional Campaigns Aimed at Young Adults?

I have chosen my question for my literature review for my Masters of Science in public health (final project) and thought social media campaigns (specifically for raising awareness of HPV...

01 January 1970 7,999 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View