I am trying to encapsulate the dye in liposomes and measure pH changes. I have seen a paper on Photochemistry and Photobiology, 2001, 74 (1), 8-13, where they mention that SNARF-1 seems to interact strongly with the membrane. I have tried similar experiments trying to encapsulate the dye in liposomes and change the pH of the bulk solution but it does not look good. They have used DOPC and egg-PC, I have also tried soybean lipids, but the response is quite similar. Has anyone come across that problem or a working protocol for SNARF-1/5F encapsulation in liposomes.

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