Differences in MALDI TOF mass from the calculate mass?

Molecular weight calculate from primary structure of my protein is 23121 but after MALDI-TOF i got 22908.90 a difference of approximately 213 dalton. Any suggestions for the observed mass difference.

22 October 2018 3,651 3 View

Why cell size increases after transfection with lipofectamine 2000?

When I transfect HeLa cells with lipofectamine 2000, cell size of 10-15% population increases within 48hrs. Transfection works quite well in our lab but I could not see the depletion of the gene....

06 June 2017 1,487 3 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

18 April 2017 3,317 3 View

Regarding refolding of protein ?

01 January 2017 1,400 12 View

Ion exchange chromatography?

I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...

28 August 2016 3,286 12 View

Suggestions for native gel electrophoresis ?

18 August 2016 2,175 8 View

Can i use Ni-NTA column itself to purify monomers from covalently cross linked dimers. ?

I have a mixture of monomer and dimer. Monomer of the protein has N terminal His tag and i did not remove the his tag after purification. I provide particular condition to my monomers so that two...

20 July 2016 5,347 3 View

Which media is best to perform plant tissue culture of bamboo seeds?

26 April 2016 149 2 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

14 May 2014 4,754 4 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

26 April 2014 9,486 25 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View