FTIR analysis for Immobilized enzyme on alginate beads?

Dear research community. I want to study FTIR spectra of Immobilized enzyme on alginate beads before and after biodegradation, do i need to grind the Immobilized enzyme on alginate beads for the...

26 October 2020 8,606 1 View

For publishing a article in journal having either ESCI or Scopus indexed. In between these two which indexing is more impactful in research domain?

Looking for this.

20 April 2020 7,588 3 View

How to search 4 word noun phrases on antconc?

Dear Linguists, I need a help regarding my dissertation relating to the search of 4-word clusters on software antconc, I am using the following model of Hyland Noun phrase + of the end of the...

14 March 2020 1,861 3 View

What are performance indicators in interoperable trust model of IoT systems?

What are performance indicators in interoperable trust model of IoT systems?

19 May 2019 4,232 1 View

How will we utilize graphene as building material?

if there is so then it may be good alternative material to electric Wires.

17 November 2018 1,356 2 View

Where can I test DSC TGA in my sample with nitrogen / inert gas flow?

My sample is an LFP precursor wherein calcination requires inert / nitrogen gas to form LFP. The problem is, in Indonesia I have not found a place to test DSC TGA with nitrogen / inert gas flow....

28 October 2018 9,352 4 View

How honey nest could negatively affect archaeological sites?

Honybee nest was found to have a negative effect on the archaeological sites e.g. affect the colors. we are trying to study the chemical composition of honey nest which could have this effect....

09 October 2018 9,922 0 View

What are the best saline (normal, PBS,DMSO) and any other to dissolve solid plant extract then give in mouse (i.p)?

i have two different solid plant extract ethanol and water to check antiepileptic activity which is the best saline to dissolve the solid plant extract to i.p in mouse. thanks & regards...

03 April 2018 543 3 View

What is the types of Statistical Model and Non Statistical Model in machine learning?

machine learning

24 February 2018 4,087 4 View

Why enzyme inhibition is decreasing with increasing concentrations of inhibitor drug?

I am testing enzyme inhibitory activity of nanoparticles but surprisingly the enzyme inhibition is decreasing with increasing concentrations of nanoparticles. Why is it so?

06 February 2018 1,785 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View