Why both Training and Validation accuracies stop improving after some epochs?

I am using data augmentation and decaying learning rate after each epoch. If I don't use data augmentation but keeping callback, then my training accuracy reaches to 99.65% but not validation....

15 December 2020 716 10 View

GAN as Data Augmentation?

Hi, I want to apply GAN as a data-augmentation technique in my model. I am bit confused regarding implementation that how could I give the generated images of GAN to my model on run-time? because...

07 December 2020 4,703 3 View

How to improve accuracy of hard classes in multiclass classification?

Hi, I am training my models to classify 10 classes. Some of the classes are easy for my models and achieve accuracy greater than 95% but some classes have very hard patterns and accuracy stops at...

03 December 2020 324 9 View

Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Can anyone please tell how to define link for Tuned mass Dampers using Sap2000/Etabs?

Dear Research scholars, I was doing my MTech thesis on pendulum based TMD and I was having trouble defining the link properties. I even followed the tutorials put on CSI website. still, I wasn't...

11 November 2020 8,309 11 View

How to solve "cz freeze python error in main script" Acp in Ansys probelm?

Greetings, I'm currently using the ANSYS 2020R1 crack version. I used this software for at least one month smoothly. But in the last few days, I'm facing this problem. I'm tired to solve this...

09 November 2020 6,431 2 View

What is the suitable optical filter for filtering 120MHz spacing combs from mode-locked laser for f-2f interferometry?

how to do f-2f interferometry from an octave spanning supercontinnum with frequency spacing 120MHz. Which would be the most suitable optical filter in this range for filtering f and 2f components...

12 October 2020 4,470 1 View

How we can design hybrid system between deep learning and swarm intelligence system or deep learning with fuzz?

Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...

08 October 2020 4,943 3 View

Why not there is common reference citation format for all journals?

Why not there is common reference citation format for all journals?

03 October 2020 8,137 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View