I'm trying to find the best method to determine H2S concentration in plasma sample from rats. I read about the methylene blue methods and was about to try this when I read about this H2S microsensor. Has anyone used it with success?
Hi.
I have used a microsensor from Unisense a few times with good results (http://www.unisense.com/H2S/). It offered different tip sizes depending on the working preparation, and I guess you could perfectly use it for plasma.
The main disadvantages were that you are required to use the sensor with the Unisense controller before getting the signal into the computer, and that the lifetime of the sensor itself (as in the instructions) was one month.
Despite this we were able to rent the acquisition equipment while buying a sensor, obtaining a lot of consistent results during that time, while maintaining the spending low. The responsiveness was fast, linearity good, and sensitivity high.
For more information you can check my article with this precise sensor (see link below).
If possible, I would skip the methylene blue method, that I used myself, and all the issues attached to it regarding the proper determination of sulfide levels.
http://www.ncbi.nlm.nih.gov/pubmed/25630260
Hi Jose,
I was wondering if you found a good way to measure H2S levels in plasma samples.
Thanks
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