Has anyone used ALDEFluor Kit in bacteria cells?

More Yonne Menezes's questions See All

How to introduce new papers in the system ?

I have now pdfs of some published papers and want to add them to the list I already have

04 January 2024 2,020 1 View

Balanced Scorecard to validate a company eficiency in the stock market?

Dear all, good everning! Do someone have already tried to perform some analysis in some company that is present in stock market using the Balanced Scorecard (BSC) to validate your eficiency? Did...

24 April 2023 5,989 0 View

Crude Oil agar plates are unevenly spread. Suggestions?

Hi All I have a student working on the enumeration and isolation of crude oil degrading bacteria following these publications:Isolation, screening, and crude oil degradation characterist... ,...

13 November 2022 3,030 16 View

Docking validation parameter?

i have done several dockings and i have my docking results, and i want to create a validation parameter for my future blind dockings does anyone have any idea how i can use my results for this?

01 November 2021 7,207 4 View

Is it possible to grow Arabidopsis thaliana without the MS medium?

Hi. I'm growing Arabidopsis thaliana on ágar medium (0.8%), aiming to extract some enzymes of the seedlings 120 hours after germination (just 5 days). Do I need to prepare the MS medium? Or can I...

08 September 2021 174 3 View

Any study on role of leisure in the life of caregivers of autism spectrum disorder?

As leisure plays major role in psychological health of caregivers i would like to know if any study done to understand the need of leisure in their life.

13 August 2021 6,339 4 View

Any computational techniques for optimization or modification of a molecule, a lead compound?

I docked a compound, and it showed good results, but I wanted to make some changes to its structure to make it more powerful, how do i do this?

16 May 2021 8,812 5 View

How to perform the molecular dynamics of the best pose and protein in NAMD / VMD?

Hey guys, I did the docking using the molegro, and now I need to perform the molecular dynamics of my best pose and my protein. I'm using Namd to do this, my question is: first of all do I need...

21 April 2021 1,018 1 View

How are materials traditionally added to a ball mill?

Doing a thesis on tape casting/slurry making for solid oxide fuel cell layers, just trying to get information on how materials are traditionally fed to the inlet of ball mills (used to make the...

06 November 2020 3,171 3 View

Genome sequenced twice in the same technology are very different. How to explain?

Hi dear all, We are studying a fungal disease and we sequenced the same fungi twice, but with different extraction times. One 11 years ago and the another one this year. The strain was extracted...

04 October 2020 428 8 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

What is the behavior of anaerobic bacteria in deep salty groundwater ?

What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks

13 July 2024 1,581 4 View