Genome sequenced twice in the same technology are very different. How to explain?

Hi dear all, We are studying a fungal disease and we sequenced the same fungi twice, but with different extraction times. One 11 years ago and the another one this year. The strain was extracted...

04 October 2020 271 8 View

Which temperature should I use in Molecular dynamics (GROMACS) for a protein found in endoplasmatic reticulum?

Hi everyone, I'm trying to perform a MD simulation for a protein found in ER. In mdp file should I set: ref-temp= 300 K or 310K? gen-temp should be the same than ref-temp or 300...

22 June 2019 2,083 6 View

How to compare two RMSD plot (frame vs frame) from gromacs?

I have two proteins. One is wild-type and the other is a mutant. The RMSD plot (nm vs time) showed me mutant is far more unstable. But, when a plot the RMSD frame vs frame graphic it seems mutant...

28 May 2019 7,260 7 View

Maintenance of PFGE electrophoresis cell during long breaks (not in use for say 3 months)!?

What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...

26 April 2016 2,895 7 View

Alkylation of aliphatic nitrogen under aqueous alkali conditions result in formation of dialkylated product?

Will a hydroxyl group in the same aliphatic molecule also form ether bond?

20 April 2016 6,801 2 View

How can I extract a urease enzyme from leguminosae with its activity ready to use?

I desire quantify urea in plants extracts!

11 September 2015 9,878 3 View

Does anyone have experience with HPLC methods for soil organic phosphorus?

Hi Everyone We are trying to reproduce Espinosa et al (1999) method but so far it has been inconsistent in the retention time of the standards. We are still working in changing the mobile phase to...

19 January 2015 2,929 3 View

Is anyone using the NEB Lambda ladder PFG Marker?

01 November 2014 5,626 11 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

14 September 2014 8,747 8 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

03 September 2014 4,789 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View