What will be the appropriate data collection method and data analysis that I can employ with may experimental research on squash germination?

I am conducting an experimental research on the germination enhancement of squash affected by mechanical, chemical and thermal scarification methods..i have researched about time- to-event method...

27 December 2020 7,133 3 View

How to conduct and interpret meta regression using prevalence rates in Stata?

I have conducted a meta-analysis of prevalence rates using metaXL. There was a large amount of heterogeneity in the studies so I now need to look at (categorical) moderating variables. I have...

15 October 2020 4,630 4 View

Are there available scales or questionnaires to measure change management?

Seeking to connect with authors who may want to share their scales or questionnaires to measure change management.

27 September 2020 374 6 View

Early amplification in both NTC and NEC in qPCR (using taqman probes)?

Hi! I've been trying to decipher what happened to some of our runs with early amplification in the NTC and NEC. This has happened to us twice now. For background, we are using a mastermix of PCR...

09 September 2020 4,559 5 View

Help with meta analysis using prevalence rates?

I have conducted a systematic review into the prevalence of victimisation in individuals with Autism. This provided a large number of relevant, high quality studies which I have synthesised into a...

14 May 2020 5,398 3 View

Why should a Journal that is Social Science Citation Indexed in the Web of Science not be listed on the Journal Citation Report (JCR)?

A Journal that is in the Web of Science (WoS) as social science citation indexd but it is not found on the list of Journal Citation Report (JCR). The website of the Journal shows also that it is...

04 May 2020 6,730 5 View

Which Augemented Reality (AR) authoring tool is easiest to use to develop an AR app by someone who does not have training in Computer Science?

I would like to introduce Augmented Reality (AR) as one of the ways to learn anatomy to our students, and I have been looking at some literature in this regard. I noted from several articles that...

15 April 2020 3,990 5 View

How to identify hypothetical proteins in bacterial genome?

I have used BLASTp and InterPro to identify the proteins in a new species of bacteriophage, which has given putative identification to roughly half. What are other ways I can identify more of the...

24 March 2020 6,706 3 View

P. aeruginosa growing with various colours?

My overnight cultures of P. aeruginosa PAO1 can range from a creamy yellow colour to a bright green - what could be causing the different pigment production? I always prepare the cultures the...

02 February 2020 7,246 3 View

Can anyone suggest me any website or source to find out green consumer /sustainable consumer data/index?

green consumer /sustainable consumer data/index?

24 January 2020 810 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View