Grace Ra

6 Questions 25 Answers 0 Followers

Questions related from Grace Ra

How do I get H295R cells to produce and secrete aldosterone?

I am having a problem getting H295R cells to produce and secrete aldosterone I am testing the aldosterone level via ELISA method. I have tried both KCL and AngII however, nothing seems to make the...

29 August 2019 6,749 0 View

How to lysis an expressing a membrane protein using a plasmid?

I have created a plasmid for my protein of interested tagged with Flag tag. The sequence looks fine but for the life of me I cant get this protein to express itself and see it using western blot (...

01 July 2019 7,210 15 View

Methods of unmasking for IHC-P ?

I am trying to calibrate an antibody which on its data sheet specifies it works for parafin IHC. On the datasheet it says it should work with PH-6 HIER method is recommended. I have used Sodium...

14 May 2019 7,852 2 View

How can I isolate exosomes from a large amount of biological fluid?

I was wondering if there is a way (not by ultra centr.) To isolate exosomes for western from around 2L of biological fluid?? Most commercial kits I saw have for 10 cc isolation tec. I am looking...

31 March 2019 3,002 9 View

How to measure amount of protein i produced from urine exosomes?

I isolated exosomes from urine (using Ultra Centr.) and want to compare different protein levels in western from the different urine types. How can I affectivly measure the amount of protein I'm...

26 March 2019 7,460 3 View

Has anyone Used a Urine Exosome purification kit and made a homemade resuspention buffer for protein recovery?

Hello, I am intrested in using a kit for isolation of Total exosomes from Urine. The kits says After the last stage of percipitation of the exosomes it says I need to buy another kit for...

27 February 2019 2,504 5 View