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Questions related from Grace Ra
I am having a problem getting H295R cells to produce and secrete aldosterone I am testing the aldosterone level via ELISA method. I have tried both KCL and AngII however, nothing seems to make the...
29 August 2019 6,744 0 View
I have created a plasmid for my protein of interested tagged with Flag tag. The sequence looks fine but for the life of me I cant get this protein to express itself and see it using western blot (...
01 July 2019 7,202 15 View
I am trying to calibrate an antibody which on its data sheet specifies it works for parafin IHC. On the datasheet it says it should work with PH-6 HIER method is recommended. I have used Sodium...
14 May 2019 7,847 2 View
I was wondering if there is a way (not by ultra centr.) To isolate exosomes for western from around 2L of biological fluid?? Most commercial kits I saw have for 10 cc isolation tec. I am looking...
31 March 2019 2,998 9 View
Hello, I am intrested in using a kit for isolation of Total exosomes from Urine. The kits says After the last stage of percipitation of the exosomes it says I need to buy another kit for...
27 February 2019 2,497 5 View