We are isolating cells from nasal lavage fluid and saving them for RNA extraction. We recover between 20,000 and 1 million cells and would like to use a lower volume if it will not affect our RNA recovery.
I want information about the actor and incident, and an assessment on the impact and response and legal issues emerging
24 May 2024 9,601 0 View
Running into an error when trying to do TD-DFT PBE0 functional and DGDZVP as basis set. Error reads as follows: 'No CIS or TD with Post-SCF methods' Input Line as follows: #p PBE0/DGDZVP...
20 May 2024 5,994 4 View
All publications since 2019. Under book chapters (2023, 20220) and 2020 under Selected Referred Journal Articles.Please add.
05 March 2024 4,634 3 View
Clear and Tinted packaging of cosmetics ,medicine and even oils used in cooking are susceptible to photo-oxidative degradation of the contents. Even food that contains lipids and fatty acids and...
27 December 2023 4,794 5 View
We have seen plastic imports like catheters from China that are toxic to patients. We have seen major legal court cases related to hernia mesh, trans vaginal mesh. Threats from polyester urethane...
24 December 2023 6,893 0 View
Current limits on both compression molded and injection molded black pigmented polyproylene is 1,000 hours at 150C using ASTM Circ.Oven on suspended plaques. The correlation of conventional O.I.T....
24 December 2023 1,015 0 View
I prepared a material and i did not get a single crystal phase. So i would like to assess the quantity or the concentration of the second phase présent in my sample. Is this possible through fullprof?
24 December 2023 5,015 5 View
The PET bottle industry use injection molded preforms to blow mold 2 liter and smaller bottles for soda and other drinks including water. Formation of toxic acetaldehyde that is reported to affect...
24 December 2023 8,757 8 View
The science of blue light wavelengths have received little attention until late. We are speaking of wavelengths from around 400-495nm this also encompasses indigo. However, the most interesting...
24 December 2023 6,444 0 View
Published within the last 5 years
27 October 2023 3,957 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View