We are isolating cells from nasal lavage fluid and saving them for RNA extraction. We recover between 20,000 and 1 million cells and would like to use a lower volume if it will not affect our RNA recovery.
I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...
21 February 2021 3,043 3 View
How can I connect IoT device/Applications to 5G network and which simulators can be used?
09 November 2020 787 5 View
03 July 2019 9,042 3 View
Hello, I am trying to create a mesh for carbon fiber layers that are placed around a cilinder (Image 5). The problem is that when I create a mesh in Abaqus (Hex elements, Structured), each...
01 July 2019 5,727 2 View
Hi there! I am trying to obtain a knock-out using Datsenko strategy. I am working with pKD4 (as a template for PCR) and the pKD46 vector. Once I obtained the linear DNA (using primers with 50 bp...
09 December 2018 2,676 10 View
I am wanting to report a Fisher's Exact as my data violated an assumption for Chi2. Can you please suggest how I report the output from SPSS in the attachment. I appreciate your guidance!
20 August 2018 7,669 4 View
Hi everyone, I have been searching and searching for the latest HIV global figures for the 15-24 years old age-group. Esp. new HIV infections & prevalence. As I am doing a research project with...
16 August 2018 3,207 1 View
This only applies to the specific Scale, the MLERS. I am writing a paper that compiles the research on this. I already have a number of studies, so if we have been in touch over the last few...
30 July 2017 3,116 3 View
18 March 2017 3,468 9 View
26 February 2017 968 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...
01 March 2021 6,985 3 View
I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...
01 March 2021 8,668 3 View
How can one improve mesh skewness, orthogonality, aspect ratio?
28 February 2021 7,730 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View