Has anyone tryed to extract DNA from arachnids for Nanopore sequencing?

Silvia Hinojosa-Alvarez @Silvia-Hinojosa-Alvarez

13 April 2018 0 7K Report

We are using the Genomic DNA kit from Qiagen, we have enough DNA and the 280-260 ratio is 1,82 . Our trouble is the A260-230 it has to be 2-2.2 and we have 1.67. I think it maybe due to amount of chitin from exoskeleton. Any suggestions?

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