Is it reasonable to use stress boundaries to simulate situations with tectonic stress in FLAC3D?

Dear all I want to build a small fault model, considering the tectonic stress, the horizontal stress is relatively high.Is this the case where you can only use the stress boundary.This is a fixed...

13 July 2020 450 3 View

Should I equilibrate protein at different temperature in molecular dynamcis simulation?

I would like to determine the structural changes of my protein at different temperature (eg. 350 K). In gromacs tutorial, the protein is equilibrated using nvt and npt under normal condition (300...

09 April 2019 9,392 3 View

Epoch by epoch analysis

Our team is planning to perform a validation of mobile device with PSG. From the literature, I have reviewed (Cook 2017), the epoch by epoch analysis was performed using MathWork. Is there any...

28 July 2017 7,179 2 View

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05 July 2017 2,232 8 View

Is there method to extract RNA of both gram positive and gram negative bacteria?

Hi, Is there a method to extract RNA of both gram positive and gram negative bacteria in their dual-species culture? I want to perform transcriptomic analysis, so I need RNA sample with high...

01 March 2017 7,922 2 View

Is there a method to differentiate pseudomonas and salmonella on visual examination?

I want to use SEM or other microscopy method to visualize the spectral organization of these two bacteria cultured together, however, they are both gram negative and rod-shaped. Is there any other...

08 March 2015 9,029 21 View

How do I isolate rat epithelial cells?

I found some protocols on the internet and I tried them out, but they don't seem to be working. I separated the cortex from the kidney and minced and then digested the tissue with HBSS buffer...

09 April 2014 9,313 1 View

How to get different paralogs assembled in the transcriptome of polyploid species?

I'm facing some problems in analyzing the sequence variation of gene paralogs in polyploid species' transcriptome, the denovo assembly using default settings of Trinity software packages produced...

05 February 2014 146 5 View

The difference between displacement and replacement

01 January 1970 1,475 2 View

What is the application of deterium excess in hydrology?

Deterium excess, or d, is defined by the following equation: d=delta(H-2)-8delta(O-18) what are the applications that have so far been reported in the literature?

01 January 1970 4,073 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View