Hello,
I'm trying to induce the hESC into the certain type of stem cell, and then embed them into the Matrigel (low-growth factor ) to generate the organoid.
But after 7 days, I haven't seen the organoid but some Strange Mass (Bright field image in pdf "1_bright_field"). It can be stained by DAPI(see pdf"2_DAPI"). In theroy, it will grow into the organoid look like in pdf"3_org",(it can be stained by some cetein antibody and the individual cell can be distinguished ).
I am sure that is not any microbial contamination.
Has this ever happened to anyone?
Thanks