Has anyone tried to manipulate mtDNA by CRISPR?

11 April 2015 1 9K Report

Just curious if anyone tried to manipulate mtDNA by CRISPR?

in our lab we are targeting zinc finger proteins to mtDNA. The biggest issue is that you'll need to engineer several mitochondrial localization signals to your targeting construct, so in this case Cas9. Also you should delete the NLS from the Cas9.

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