Has anyone tried to culture Sf9 cells (suspension) in YPD medium?

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Attributes to poverty in uganda?

citations

27 March 2024 5,206 1 View

Does Anyone know how much methanol required for waste water treatment for 1 MGD Facility ?

I need information of methanol requirement for 1 MGD Waste Water Treatment

21 March 2024 8,516 3 View

Examine the use and abuse of the internet by university students?

How university student use and abuse the internet

11 March 2024 1,578 1 View

How to update my biographical data ?

i WOULD LIKE TO UPDATE MY BIOGRAFICAL INFORMATION . For instance I am not anymore involved ad the Institution and department Ghent UniversityMUSEUM . i TRIED TO CORRECT THIS BUT WITHOUT SUCCES.

05 January 2024 1,350 2 View

Dear researchers & fellow academics, i humbly ask is international journal of research and scientific innovation (ijrsi) (ISSN 2321-2705) predatory?

i mean...i can't seem to find it in the Beall's list of potential predatory publishers and journals. The website interface of the ijrsi seem suspicious, and i've got email notified me that if i...

27 December 2023 3,081 5 View

Can We Make a Better Thermal Insulator?

In a well-built apartment building, each apartment has its own walls, and there is an air gap between the walls of adjacent apartments, which provides excellent sound isolation. Could a similar...

17 November 2023 3,174 9 View

What is the brand of my LAPTOP?

Include the make and model.

14 October 2023 2,385 3 View

LPS- free M1 macrophage differentiation?

Everyone knows that LPS is pretty artificial, especially if we're talking M1 macrophages for a cancer setting. I've heard something about using TNFa instead of LPS, but darn if I can find it. Does...

17 May 2023 9,403 2 View

Old Quotes On Ethnic Intelligence?

People have noticed centuries and millennia ago that ethnic groups and peoples differ from each other. I am looking for quotations from ancient times that contain judgments about the intelligence...

02 May 2023 7,964 4 View

UHPLC instrument issues with NH4PF6 and KPF6 (100 mM, pH 3.5) as buffer salts/additive in the mobile phase?

I am developing a UHPLC method for content and purity for a peptide based on 100mM KPF6 and 100mM NH4PF6 pH 3.5. TFA as an ion pair reagent does not give the required separation performance. My...

20 April 2023 9,370 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View